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KMID : 0545120070170040600
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 4 p.600 ~ p.603
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Effect of Ionic Liquid on the Kinetics of Peroxidase Catalysis
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Lee Yoon-Mi
Kwon O-Yul
Yoo Ik-Keun
Ryu Keun-Garp
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Abstract
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The effect of a water-miscible ionic liquid, 1- butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), on the horseradish peroxidase (HRP)-catalyzed oxidation of 2-methoxyphenol (guaiacol) with hydrogen peroxide (H2O2) was investigated. HRP maintains its high activity in the aqueous mixtures containing various concentrations of the ionic liquid and even in 90% (v/v) ionic liquid. In order to minimize the effect of solution viscosity on the kinetic constants of HRP catalysis, the enzymatic reactions in the subsequent kinetic study were performed in water-ionic liquid mixtures containing 25% (v/v) ionic liquid at maximum. As the concentration of [BMIM][BF4] increased for the oxidation of guaiacol by HRP, the Km value increased with a slight decrease in the kcat value: The Km value increased from 2.8 mM in 100% (v/v) water to 22.5 mM in 25% (v/v) ionic liquid, indicating that ionic liquid significantly weakens the binding affinity of guaiacol to HRP.
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KEYWORD
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Ionic liquid, kinetics, peroxidase, guaiacol
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